ACTN2 Antibody / Alpha Actinin 2 / Sarcomeric Alpha Actinin

NSJ Bioreagents
Product Code: NSJ-V7530
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7530-20UG20 ug£264.00
Quantity:
NSJ-V7530-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SAMA-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the ACTN2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
IHC staining of FFPE human skeletal muscle tissue with Sarcomeric Alpha Actinin antibody (clone SAMA-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
2 / 2
Western blot testing of

IHC staining of FFPE human skeletal muscle tissue with Sarcomeric Alpha Actinin antibody (clone SAMA-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
Western blot testing of

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The optimal dilution of the ACTN2 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The ACTN2 gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.05% sodium azide
Immunogen:
Amino acids 557-692 from the human protein were used as the immunogen for this ACTN2 antibody.
Limitation:
This ACTN2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P35609