SERBP1 Antibody / PAI-RBP1

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NSJ Bioreagents
Product Code: NSJ-V8445
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8445-20UG20 ug£264.00
Quantity:
NSJ-V8445-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SERBP1/3494
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the SERBP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
Western blot testing of human 1) K562 and 2) PC-3 cell lysate with SERBP1 antibody. Predicted molecular weight ~45 kDa but observed at 45-60 kDa.
2 / 4
IHC staining of FFPE human urothelial carcinoma with SERBP1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human kidney with SERBP1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERBP1 antibody. These results demonstrate the foremost specificity of the SERBP1/3494 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of human 1) K562 and 2) PC-3 cell lysate with SERBP1 antibody. Predicted molecular weight ~45 kDa but observed at 45-60 kDa.
IHC staining of FFPE human urothelial carcinoma with SERBP1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with SERBP1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERBP1 antibody. These results demonstrate the foremost specificity of the SERBP1/3494 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the SERBP1 antibody should be determined by the researcher.
Description:
SERBP1 is a membrane-associated protein that localizes to the nucleus, the perinuclear region of the cytoplasm and the plasma membrane. It is believed to play a role in the regulation of mRNA stability, as it specifically binds to the CRS (cyclic nucleotide-responsive sequence) motif of the PAI-1 mRNA and acts to stabilize the mRNA and regulate its expression. In addition, SERBP1 interacts with Mi2-s antiapoptotic action in ovarian cell types. SERBP1 is overexpressed in ovarian cancer, suggesting a possible role in tumorigenesis and tumor metastasis.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 3-139 from the human protein was used as the immunogen for the SERBP1 antibody.
Limitation:
This SERBP1 antibody is available for research use only.
Localization:
Cytoplasm, nucleus, perinuclear regions
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q8NC51