CD15 Antibody / SSEA-1

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NSJ Bioreagents
Product Code: NSJ-V7799
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7799-20UG20 ug£264.00
Quantity:
NSJ-V7799-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgM, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM119
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of human monocytes with CD15 antibody (clone SPM119); Green=isotype control, Red= CD15 antibody.
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Flow cytometry testing of human U937 cells with CD15 antibody (clone SPM119); Red=isotype control, Blue= CD15 antibody.
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Immunofluorescent staining of human U937 cells with CD15 antibody (clone SPM119, green) and Reddot nuclear stain (red).
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IHC staining of FFPE Hodgkins lymphoma with CD15 antibody (clone SPM119). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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Flow cytometry testing of human monocytes with CD15 antibody (clone SPM119); Green=isotype control, Red= CD15 antibody.
Flow cytometry testing of human U937 cells with CD15 antibody (clone SPM119); Red=isotype control, Blue= CD15 antibody.
Immunofluorescent staining of human U937 cells with CD15 antibody (clone SPM119, green) and Reddot nuclear stain (red).
IHC staining of FFPE Hodgkins lymphoma with CD15 antibody (clone SPM119). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Human U937 cells were used as the immunogen for the CD15 antibody.
Limitation:
This CD15 antibody is available for research use only.
Purity:
PEG precipitation
Species Reactivity :
Human
Uniprot #:
P22083