Cytokeratin 20 Antibody / CK20

Contact us
NSJ Bioreagents
Product Code: NSJ-V8019
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8019-20UG20 ug£264.00
Quantity:
NSJ-V8019-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM140
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone SPM140). Predicted molecular weight ~46 kDa.
2 / 3
IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone SPM140). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone SPM140) as confirmation of integrity and purity.
❮❯

Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone SPM140). Predicted molecular weight ~46 kDa.
IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone SPM140). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone SPM140) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
his MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
Limitation:
This Cytokeratin 20 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P35900