c-Myc Antibody
Code | Size | Price |
---|
NSJ-V2745-20UG | 20 ug | £264.00 |
Quantity:
NSJ-V2745-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM237
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Myc antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Images
Documents
Further Information
Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
Application Note:
Optimal dilution of the Myc antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus were used as the immunogen for the Myc antibody.
Limitation:
This Myc antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P01106