Cytokeratin 14 Antibody / CK14 / KRT14

Contact us
NSJ Bioreagents
Product Code: NSJ-V9058
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9058-20UG20 ug£264.00
Quantity:
NSJ-V9058-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG3, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM263
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the anti-Cytokeratin 14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with anti-Cytokeratin 14 antibody (clone SPM263).
2 / 4
IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with anti-Cytokeratin 14 antibody (clone SPM263).
3 / 4
Immunofluorescent staining of fixed human A549 cells with anti-Cytokeratin 14 antibody (clone SPM263, green) and Reddot nuclear stain (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free anti-Cytokeratin 14 antibody (clone SPM263) as confirmation of integrity and purity.
❮❯

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with anti-Cytokeratin 14 antibody (clone SPM263).
IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with anti-Cytokeratin 14 antibody (clone SPM263).
Immunofluorescent staining of fixed human A549 cells with anti-Cytokeratin 14 antibody (clone SPM263, green) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free anti-Cytokeratin 14 antibody (clone SPM263) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A 15 amino acid peptide from the C-terminus of human keratin 14 was used as the immunogen for this anti-Cytokeratin 14 antibody.
Limitation:
This anti-Cytokeratin 14 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P02533