Cytokeratin 18 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9040
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9040-20UG20 ug£264.00
Quantity:
NSJ-V9040-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM265
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
Immunofluorescent staining of permeabilized human HeLa cells with Cytokeratin 18 antibody (clone SPM265, green) and Reddot nuclear stain (red).
2 / 4
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (clone SPM265).
3 / 4
Western blot testing of human 1) A431 and 2) HeLa lysate using Cytokeratin 18 antibody (clone SPM265). Predicted molecular weight ~48 kDa.
4 / 4
Western blot testing of human HCT116 cell lysate using Cytokeratin 18 antibody (clone SPM265). Predicted molecular weight ~48 kDa.
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Immunofluorescent staining of permeabilized human HeLa cells with Cytokeratin 18 antibody (clone SPM265, green) and Reddot nuclear stain (red).
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (clone SPM265).
Western blot testing of human 1) A431 and 2) HeLa lysate using Cytokeratin 18 antibody (clone SPM265). Predicted molecular weight ~48 kDa.
Western blot testing of human HCT116 cell lysate using Cytokeratin 18 antibody (clone SPM265). Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
The human breast cancer cell line PMC-42 was used as the immunogen for this Cytokeratin 18 antibody.
Limitation:
This Cytokeratin 18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P05783