RPA32 Antibody / Replication Protein A2

NSJ Bioreagents
Product Code: NSJ-V8066
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8066-20UG20 ug£264.00
Quantity:
NSJ-V8066-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM316
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store the Replication Protein A2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human samples with Replication Protein A2 antibody (clone SPM316). Expected molecular weight ~32 kDa.

Western blot testing of human samples with Replication Protein A2 antibody (clone SPM316). Expected molecular weight ~32 kDa.

Further Information

Application Details :
Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human full-length protein was used as the immunogen for this Replication Protein A2 antibody.
Limitation:
This Replication Protein A2 antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Human