CD15 Antibody / SSEA-1

NSJ Bioreagents
Product Code: NSJ-V2523
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2523-20UG20 ug£264.00
Quantity:
NSJ-V2523-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgM, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM490
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the SSEA-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of human U937 cells with Calponin antibody (clone SPM490, green) and Reddot nuclear stain (red).~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with SSEA-1 antibody (clone SPM490).

Immunofluorescent staining of human U937 cells with Calponin antibody (clone SPM490, green) and Reddot nuclear stain (red).~
IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with SSEA-1 antibody (clone SPM490).

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the SSEA-1 antibody should be determined by the researcher.
Description:
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Myelomonocytic leukemia cells were used as the immunogen for the SSEA-1 antibody.
Limitation:
This SSEA-1 antibody is available for research use only.
Localization:
Cell surface and granular paranuclear
Purity:
PEG precipitation
Species Reactivity :
Human
Uniprot #:
P22083