CD20 Antibody
| Code | Size | Price |
|---|
| NSJ-V2397-0.1ML | 0.1 ml | £264.00 |
Quantity:
| NSJ-V2397-0.5ML | 0.5 ml | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM494
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Documents
Further Information
Application Details :
Flow cytometry: 2-5ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Format :
Bioreactor concentrate
Formulation :
Bioreactor concentrate with 0.05% sodium azide
Immunogen:
Human tonsil B cells were used as the immunogen for this CD20 antibody.
Limitation:
This CD20 antibody is available for research use only.
Localization:
Predominantly cell surface with some cytoplasmic
Purity:
Unpurified high titer supernatant
Species Reactivity :
Human
Uniprot #:
P11836