Folate Hydrolase 1 Antibody / FOLH1 / PSMA

NSJ Bioreagents
Product Code: NSJ-V7739
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7739-20UG20 ug£264.00
Quantity:
NSJ-V7739-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM500
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Folate Hydrolase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Western blot testing of human LNCaP cell lysate with Folate Hydrolase 1 antibody. Predicted molecular weight ~100 kDa.
2 / 3
IHC testing of FFPE human prostate cancer with Folate Hydrolase 1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Folate Hydrolase 1 antibody as confirmation of integrity and purity.

Western blot testing of human LNCaP cell lysate with Folate Hydrolase 1 antibody. Predicted molecular weight ~100 kDa.
IHC testing of FFPE human prostate cancer with Folate Hydrolase 1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Folate Hydrolase 1 antibody as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 232-433 from the human protein was used as the immunogen for this Folate Hydrolase 1 antibody.
Limitation:
This Folate Hydrolase 1 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q04609