CD44 Antibody / HCAM

NSJ Bioreagents
Product Code: NSJ-V9105
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9105-20UG20 ug£264.00
Quantity:
NSJ-V9105-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM544
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the anti-CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human HeLa cell lysate with anti-CD44 antibody (clone SPM544). Predicted molecular weight ~81 kDa.~
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IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD44 antibody (clone SPM544).

Western blot testing of human HeLa cell lysate with anti-CD44 antibody (clone SPM544). Predicted molecular weight ~81 kDa.~
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD44 antibody (clone SPM544).

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Stimulated human leukocytes were used as the immunogen for this anti-CD44 antibody.
Limitation:
This anti-CD44 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16070