p53 Antibody / TP53

Contact us
NSJ Bioreagents
Product Code: NSJ-V9089
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9089-20UG20 ug£264.00
Quantity:
NSJ-V9089-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM589
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
Western blot testing of human HeLa cell lysate with anti-p53 antibody (clone SPM589). Expetected molecular weight ~53 kDa.~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-p53 antibody (clone SPM589).
❮❯

Western blot testing of human HeLa cell lysate with anti-p53 antibody (clone SPM589). Expetected molecular weight ~53 kDa.~
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-p53 antibody (clone SPM589).

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
Limitation:
This anti-p53 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04637