dsDNA Antibody / Double Stranded DNA
| Code | Size | Price |
|---|
| NSJ-V3081-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V3081-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG3, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM603
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Double Stranded DNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.
Limitation:
This Double Stranded DNA antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Not Known