EGF Receptor / EGFR Antibody
| Code | Size | Price |
|---|
| NSJ-V2486-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V2486-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM622
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the anti-EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details :
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the anti-EGFR antibody should be determined by the researcher.
1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human EGFR purified from A431 cells was used as the immunogen for the anti-EGFR antibody.
Limitation:
This anti-EGFR antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P00533