HSP60 Antibody

NSJ Bioreagents
Product Code: NSJ-V7117
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7117-20UG20 ug£264.00
Quantity:
NSJ-V7117-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SRPR60
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone SRPR60, green) and Reddot nuclear stain (red).~
2 / 2
IHC testing of FFPE human bladder carcinoma and HSP60 antibody (clone SRPR60). Staining of formalin-fixe

Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone SRPR60, green) and Reddot nuclear stain (red).~
IHC testing of FFPE human bladder carcinoma and HSP60 antibody (clone SRPR60). Staining of formalin-fixe

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the HSP60 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human HSPD1 protein was used as the immunogen for the HSP60 antibody.
Limitation:
This HSP60 antibody is available for research use only.
Localization:
Cytoplasm (mitochondria)
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P10809