Arginase 1 Antibody

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

A C-terminal recombinant protein fragment from ARG1 was used as the immunogen for the Arginase 1 antibody.

This Arginase 1 antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

Human

P05089