Topoisomerase II alpha Antibody / TOP2A

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NSJ Bioreagents
Product Code: NSJ-V3443
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3443-20UG20 ug£264.00
Quantity:
NSJ-V3443-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TOP2A/1361
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
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Western blot testing of human HeLa cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells with Topoisomerase II alpha antibody (green, clone TOP2A/1361) and Phalloidin (red).
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Topoisomerase II alpha antibody (clone TOP2A/1361). These results demonstrate the foremost specificity of the TOP2A/1361 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
Western blot testing of human HeLa cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
Immunofluorescent staining of PFA-fixed human HeLa cells with Topoisomerase II alpha antibody (green, clone TOP2A/1361) and Phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Topoisomerase II alpha antibody (clone TOP2A/1361). These results demonstrate the foremost specificity of the TOP2A/1361 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.
Description:
This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
7153
Immunogen:
Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.
Limitation:
This Topoisomerase II alpha antibody is available for research use only.
Localization:
Nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P11388