p53 Antibody / TP53

Contact us
NSJ Bioreagents
Product Code: NSJ-V3952
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3952-20UG20 ug£264.00
Quantity:
NSJ-V3952-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TP53/1739
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
IHC testing of FFPE human colon carcinoma stained with p53 antibody (clone TP53/1739). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
Analysis of HuProt(TM) microa
❮❯

IHC testing of FFPE human colon carcinoma stained with p53 antibody (clone TP53/1739). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.~
Analysis of HuProt(TM) microa

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the p53 antibody should be determined by the researcher.
Description:
Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full length human protein was used as the immunogen for the p53 antibody.
Limitation:
This p53 antibody is available for research use only.
Localization:
Nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04637