TLR9 Antibody

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NSJ Bioreagents
Product Code: NSJ-N1014
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-N1014-25UG25 ug£254.00
Quantity:
NSJ-N1014-100UG100 ug£450.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TR9541
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TLR9 antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human samples with TLR9 antibody at 2ug/ml.
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Western blot testing of mouse samples with TLR9 antibody at 2ug/ml.
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IHC testing of FFPE human stomach tissue with TLR9 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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FACS testing of human PBMC (lymphocytes) with TLR9 antibody at 0.5ug/10^6 cells. Green: isotype control; Red: TLR9 antibody.
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Western blot testing of human samples with TLR9 antibody at 2ug/ml.
Western blot testing of mouse samples with TLR9 antibody at 2ug/ml.
IHC testing of FFPE human stomach tissue with TLR9 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
FACS testing of human PBMC (lymphocytes) with TLR9 antibody at 0.5ug/10^6 cells. Green: isotype control; Red: TLR9 antibody.

Documents

Further Information

Application Details :
Western blot: 2-4ug/ml,Flow Cytometry: 1ug/million cells,Immunohistochemistry (FFPE): 5ug/ml
Application Note:
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Buffer:
1X PBS, pH 7.4
Description:
The mammalian immune system is composed of two components: innate immunity and adaptive immunity. Toll-like receptors (TLRs) are an essential part of the innate immune system. Through recognition of pathogen-associated molecular patterns (PAMPs), TLRs mount an immune defense against pathogens. There are 10 mammalian TLRs (TLR1-10). Unmethylated CpG sequences in bacterial genomes and viral DNA molecules activates TLR9, leading to the inflammatory response.
Format :
Purified
Formulation :
0.5 mg/ml antibody in PBS with 0.1 mg/ml BSA (US source) and 0.05% sodium azide
Gene ID #:
54106
Immunogen:
A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody.
Limitation:
This TLR9 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G Affinity Chromatography
Species Reactivity :
Human, Mouse