Thymidylate Synthase Antibody

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NSJ Bioreagents
Product Code: NSJ-V3515
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3515-20UG20 ug£264.00
Quantity:
NSJ-V3515-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TYMS/1884
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the TYMS antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of PFA-fixed human Ramos cells with TYMS antibody cocktail (green, clone TYMS/1884) and Phalloidin (red).
2 / 5
IHC testing of FFPE human tonsil tissue with TYMS antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 5
Flow cytometry testing of PFA-fixed human Ramos cells with TYMS antibody (clone TYMS/1884); Red=isotype control, Blue= TYMS antibody.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TYMS antibody (clone TYMS/1884). These results demonstrate the foremost specificity of the TYMS/1884 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free TYMS antibody (clone TYMS/1884) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human Ramos cells with TYMS antibody cocktail (green, clone TYMS/1884) and Phalloidin (red).
IHC testing of FFPE human tonsil tissue with TYMS antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of PFA-fixed human Ramos cells with TYMS antibody (clone TYMS/1884); Red=isotype control, Blue= TYMS antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TYMS antibody (clone TYMS/1884). These results demonstrate the foremost specificity of the TYMS/1884 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free TYMS antibody (clone TYMS/1884) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the TYMS antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS, TYMS). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A recombinant human parital protein corresponding to amino acids 60-174 was used as the immunogen for this TYMS antibody.
Limitation:
This TYMS antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04818