von Willebrand Factor Antibody / vWF

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Variations in protocols, secondaries and substrates may require the von Willebrand Factor antibody to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

von Willebrand Factor (vWF) is a glycoprotein produced by bone marrow cells and endothelial cells lining the inside surface of blood vessels. Its primary function is platelet adhesion, binding to Factor VIII, collagen and platelets, to coagulate blood at the site of wounding. The enzyme VWFCP, or vWF-cleaving protease, facilitates clotting by cutting the protein into subunits, increasing its binding capacity.

Deficiency or dysfunction of the protein increases the tendency of wounds to bleed, or to bleed more. Over 300 gene mutations have been identified and classified into three types. Type 1 von Willebrand Factor disease is characterized by reduced amounts in the bloodstream, Type 2 by reduced binding ability and Type 3 by a nonfunctional protein.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

7450

A recombinant human protein fragment was used as the immunogen for this von Willebrand Factor antibody.

This von Willebrand Factor antibody is available for research use only.

Cytoplasmic

Protein G purified von Willebrand Factor antibody

Human