ZAP70 Antibody

ELISA: 2-4ug/ml for coating (order Ab without BSA),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Optimal dilution of the recombinant ZAP70 antibody should be determined by the researcher.

Zeta-associated protein-70 (ZAP-70) is a member of the Syk family of tyrosine kinases, a group of proteins that attach to the zeta chain components of T-cell receptors to signal downstream events involved in the regulation of cell function, proliferation, and death. Research suggests that the ZAP-70 protein may also play an important role in natural killer (NK) cell activation and early B-cell development; however, it is not expressed in most normal mature B-cells. Expression of ZAP-70 has been reported in various lymphomas, including mantle cell lymphoma, small lymphocytic lymphoma and marginal zone lymphoma. During thymocyte development, ZAP-70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP-70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

A portion of amion acids 200-350 from the human protein was used as the immunogen for the recombinant ZAP70 antibody.

This recombinant ZAP70 antibody is available for research use only.

Cell surface, cytoplasm

Protein A affinity chromatography

Human

P43403