HER2 Antibody / ErbB2 / Extracellular region

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NSJ Bioreagents
Product Code: NSJ-V8632
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8632-20UG20 ug£264.00
Quantity:
NSJ-V8632-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit
Antibody Clonality: Monoclonal
Antibody Clone: ZR5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5); Red=isotype control, Blue= HER2 antibody.
4 / 4
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5, green) and Phalloidin (red).
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IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5); Red=isotype control, Blue= HER2 antibody.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5, green) and Phalloidin (red).

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.
Description:
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the recombinant HER2 antibody.
Limitation:
This recombinant HER2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
IDP04626