CREB Antibody

NSJ Bioreagents
Product Code: NSJ-F41641
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41641-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41641-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the CREB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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CREB antibody western blot analysis in HT29 lysate
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Fluorescent image of A549 cell stained with CREB antibody. Alexa Fluor 488 (green) secondary was used (1:400, 50 min at 37oC). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin. Immunoreactivity is localized to the nucleus.
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CREB antibody flow cytometric analysis of A549 cells (green) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

CREB antibody western blot analysis in HT29 lysate
Fluorescent image of A549 cell stained with CREB antibody. Alexa Fluor 488 (green) secondary was used (1:400, 50 min at 37oC). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin. Immunoreactivity is localized to the nucleus.
CREB antibody flow cytometric analysis of A549 cells (green) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 105-132 from the human protein was used as the immunogen for this CREB antibody.
Limitation:
This CREB antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine
Uniprot #:
P16220