HMGA1 Antibody

NSJ Bioreagents
Product Code: NSJ-F51525
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51525-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51525-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the HMGA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent image of A549 cell stained with HMGA1 antibody at 1:25. HMGA1 immunoreactivity is localized to the nucleus.
2 / 3
HMGA1 antibody western blot analysis in K562 lysate
3 / 3
HMGA1 antibody flow cytometric analysis of MCF-7 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Fluorescent image of A549 cell stained with HMGA1 antibody at 1:25. HMGA1 immunoreactivity is localized to the nucleus.
HMGA1 antibody western blot analysis in K562 lysate
HMGA1 antibody flow cytometric analysis of MCF-7 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the HMGA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 64-93 from the human protein was used as the immunogen for this HMGA1 antibody.
Limitation:
This HMGA1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Hamster
Uniprot #:
P17096