UHRF1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F51386
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51386-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51386-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the UHRF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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UHRF1 antibody western blot analysis of HeLa lysate. Predicted molecular weight ~ 88 kDa.
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Fluorescent confocal image of HeLa cells stained with UHRF1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. UHRF1 immunoreactivity is localized to the nucleus.
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UHRF1 antibody flow cytometry analysis of HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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UHRF1 antibody western blot analysis of HeLa lysate. Predicted molecular weight ~ 88 kDa.
Fluorescent confocal image of HeLa cells stained with UHRF1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. UHRF1 immunoreactivity is localized to the nucleus.
UHRF1 antibody flow cytometry analysis of HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the UHRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 229-257 from the human protein was used as the immunogen for this UHRF1 antibody.
Limitation:
This UHRF1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q96T88