ADA Antibody

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NSJ Bioreagents
Product Code: NSJ-F41596
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41596-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41596-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the ADA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human duodenum tissue with ADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
ADA antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
Western blot testing of human 1) MOLT-4, 2) Jurkat, 3) HeLa, 4) HL-60 and 5) DU145 cell lysate with ADA antibody. Expected molecular weight ~41 kDa.
4 / 4
ADA antibody western blot analysis in HL-60 lysate. Expected molecular weight ~41 kDa.
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IHC testing of FFPE human duodenum tissue with ADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
ADA antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of human 1) MOLT-4, 2) Jurkat, 3) HeLa, 4) HL-60 and 5) DU145 cell lysate with ADA antibody. Expected molecular weight ~41 kDa.
ADA antibody western blot analysis in HL-60 lysate. Expected molecular weight ~41 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the ADA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Adenosine deaminase (ADA) catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 287-314 from the human protein was used as the immunogen for this ADA antibody.
Limitation:
This ADA antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00813