KLF4 Antibody

NSJ Bioreagents
Product Code: NSJ-F41813
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41813-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41813-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
KLF4 antibody western blot analysis in (1) A431 and (2) MCF-7 lysate. Predicted molecular weight: 50-60 kDa + ~75 kDa
2 / 3
Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:200. KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
3 / 3
KLF4 antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

KLF4 antibody western blot analysis in (1) A431 and (2) MCF-7 lysate. Predicted molecular weight: 50-60 kDa + ~75 kDa
Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:200. KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
KLF4 antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 394-421 from the human protein was used as the immunogen for this KLF4 antibody.
Limitation:
This KLF4 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O43474