XRCC5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F41815
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41815-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41815-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the XRCC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of XRCC5 antibody with HeLa cells followed by Alexa Fluor 488-conjugated secondary (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used to stain the nuclei (blue).
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XRCC5 antibody western blot analysis in human placenta tissue lysate
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XRCC5 antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of XRCC5 antibody with HeLa cells followed by Alexa Fluor 488-conjugated secondary (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used to stain the nuclei (blue).
XRCC5 antibody western blot analysis in human placenta tissue lysate
XRCC5 antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the XRCC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 424-450 from the human protein was used as the immunogen for this XRCC5 antibody.
Limitation:
This XRCC5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P13010