SMAD2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50346
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50346-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50346-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:200. Note the highly specific localization of the SMAD2 to the nucleus.~
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Western blot analysis of SMAD2 antibody and NCI-H460 lysate. Predicted molecular weight:
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Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:200. Note the highly specific localization of the SMAD2 to the nucleus.~
Western blot analysis of SMAD2 antibody and NCI-H460 lysate. Predicted molecular weight:

Documents

Further Information

Application Details :
Immunofluorescence: 1:200,Western blot: 1:1000
Application Note:
Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 201-230 from the human protein was used as the immunogen for this SMAD2 antibody.
Limitation:
This SMAD2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Zebrafish
Uniprot #:
Q15796