Anti-E Cadherin Antibody

NSJ Bioreagents
Product Code: NSJ-F44157
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44157-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44157-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the anti-E Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of anti-E Cadherin antibody and A375 lysate
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Western blot analysis of anti-E Cadherin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (2).
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Anti-E Cadherin antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of anti-E Cadherin antibody and A375 lysate
Western blot analysis of anti-E Cadherin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (2).
Anti-E Cadherin antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the anti-E Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. E Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 833-862 from the human protein was used as the immunogen for this anti-E Cadherin antibody.
Limitation:
This anti-E Cadherin antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P12830