CD27 Antibody

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NSJ Bioreagents
Product Code: NSJ-F43213
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F43213-0.08ML0.08 ml£260.00
Quantity:
NSJ-F43213-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the CD27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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CD27 antibody western blot analysis in Ramos lysate. Predicted molecular weight ~29 kDa.
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Western blot analysis of CD27 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the CD27 gene.
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CD27 antibody flow cytometric analysis of Ramos cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.
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CD27 antibody western blot analysis in Ramos lysate. Predicted molecular weight ~29 kDa.
Western blot analysis of CD27 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the CD27 gene.
CD27 antibody flow cytometric analysis of Ramos cells (right histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (left histogram). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CD27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 196-225 from the human protein was used as the immunogen for this CD27 antibody.
Limitation:
This CD27 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P26842