CD4 Antibody

NSJ Bioreagents
Product Code: NSJ-F44339
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44339-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44339-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the CD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
CD4 antibody flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 3
Western blot analysis of CD4 antibody and CEM lysate; Expected size: 40~60 kDa depending on glycosylation level
3 / 3
Western blot analysis of CD4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (2).

CD4 antibody flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of CD4 antibody and CEM lysate; Expected size: 40~60 kDa depending on glycosylation level
Western blot analysis of CD4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (2).

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 58-86 from the human protein was used as the immunogen for this CD4 antibody.
Limitation:
This CD4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P01730