CDC20 Antibody

NSJ Bioreagents
Product Code: NSJ-F51546
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51546-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51546-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the CDC20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of CDC20 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the CDC20 gene.
2 / 3
Western blot analysis of CDC20 antibody and HeLa lysate.
3 / 3
CDC20 antibody flow cytometry analysis of HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of CDC20 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the CDC20 gene.
Western blot analysis of CDC20 antibody and HeLa lysate.
CDC20 antibody flow cytometry analysis of HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CDC20 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 57-84 from the human protein was used as the immunogen for this CDC20 antibody.
Limitation:
This CDC20 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Pig
Uniprot #:
Q12834