PPARG Antibody

NSJ Bioreagents
Product Code: NSJ-F49368
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49368-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49368-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the PPARG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
PPARG antibody flow cytometric analysis of HepG2 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 4
PPARG antibody flow cytometric analysis of human HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
Western blot testing of human 1) fetal liver, 2) lung and 3) placenta lysate with PPARG antibody at 1:2000. Expected molecular weight: 54-57 kDa.
4 / 4
Western blot testing of human lung lysate with PPARG antibody at 1:2000. Expected molecular weight: 54-57 kDa.

PPARG antibody flow cytometric analysis of HepG2 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
PPARG antibody flow cytometric analysis of human HeLa cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of human 1) fetal liver, 2) lung and 3) placenta lysate with PPARG antibody at 1:2000. Expected molecular weight: 54-57 kDa.
Western blot testing of human lung lysate with PPARG antibody at 1:2000. Expected molecular weight: 54-57 kDa.

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PPARG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PPARG is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity). [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this PPARG antibody.
Limitation:
This PPARG antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P37231