GLI2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F52545
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52545-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52545-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the GLI2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from (1) 293 and (2) Jurkat cell line using GLI2 antibody at 1:1000. Predicted molecular weight: 86~168 kDa (multiple isoforms)(1).
2 / 3
Flow cytometric analysis of HeLa cells using GLI2 antibody (green) compared to an <a href=../search_result.php?search_txt=n1001>isotype control of rabbit IgG</a> (blue). Ab diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
3 / 3
Fluorescent image of HeLa cells stained with GLI2 antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Western blot analysis of lysate from (1) 293 and (2) Jurkat cell line using GLI2 antibody at 1:1000. Predicted molecular weight: 86~168 kDa (multiple isoforms)(1).
Flow cytometric analysis of HeLa cells using GLI2 antibody (green) compared to an <a href=../search_result.php?search_txt=n1001>isotype control of rabbit IgG</a> (blue). Ab diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
Fluorescent image of HeLa cells stained with GLI2 antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Flow Cytometry: 1:25,Western blot: 1:1000
Application Note:
Titration of the GLI2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. GLI-2 exists as five alternatively spliced isoforms of 133 kDa (isoform 1), 132 (2), 88 (3), 86 (4) and 168 kDa (isoform 5).
Description:
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Is involved in the smoothened (SHH) signaling pathway.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This GLI2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1287-1321 amino acids from the C-terminal region of human GLI2.
Limitation:
This GLI2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P10070