NQO1 Antibody

NSJ Bioreagents
Product Code: NSJ-F50335
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50335-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50335-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the NQO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of NQO1 antibody and NCI-H460 lysate
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NQO1 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of NQO1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Western blot analysis of NQO1 antibody and NCI-H460 lysate
NQO1 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of NQO1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the NQO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NQO1 is a member of the NAD(P)H dehydrogenase(quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 118-144 from the human protein was used as the immunogen for this NQO1 antibody.
Limitation:
This NQO1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P15559