NRAS Antibody

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NSJ Bioreagents
Product Code: NSJ-F50733
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50733-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50733-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the NRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of NRAS antibody and CEM lysate. Predicted molecular weight ~21 kDa.
2 / 4
Western blot analysis of NRAS antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the NRAS gene (2).
3 / 4
NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
Confocal immunofluorescent analysis of NRAS antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of NRAS antibody and CEM lysate. Predicted molecular weight ~21 kDa.
Western blot analysis of NRAS antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the NRAS gene (2).
NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of NRAS antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 72-101 from the human protein was used as the immunogen for this NRAS antibody.
Limitation:
This NRAS antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Chicken, Mouse, Pig, Rat
Uniprot #:
P01111