p38 Antibody (MAPK14)

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NSJ Bioreagents
Product Code: NSJ-F50496
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50496-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50496-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the p38 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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p38 antibody used in western blot to detect p38 in Jurkat cell lysate
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Western blot analysis of p38 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (2).
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p38 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of p38 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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p38 antibody used in western blot to detect p38 in Jurkat cell lysate
Western blot analysis of p38 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (2).
p38 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of p38 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the p38 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
P38 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 295-324 from the human protein was used as the immunogen for this p38 antibody.
Limitation:
This p38 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
Q16539