NANOG Antibody
| Code | Size | Price |
|---|
| NSJ-F53329-0.08ML | 0.08 ml | £260.00 |
Quantity:
| NSJ-F53329-0.4ML | 0.4 ml | £502.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Western blot: 1:1000-2000
Application Note:
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This NANOG antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 155-189 amino acids from the Central region of human NANOG.
Limitation:
This NANOG antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9H9S0