Neutrophil elastase Antibody

Western blot: 1:1000

Titration of the Neutrophil elastase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 13-39 from the human protein was used as the immunogen for this Neutrophil elastase antibody.

This Neutrophil elastase antibody is available for research use only.

Antigen affinity

Human

P08246