Phospho-Src Antibody (pY215)

NSJ Bioreagents
Product Code: NSJ-F48452
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48452-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48452-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot the phospho-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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FG pancreatic carcinoma cell lines stably expressing vector alone (FG-V), the b3 integrin subunit (FG-b3), or a b3 truncation mutant (FG-759x) probed with phospho-Src antibody. Ab was diluted 1:500.

FG pancreatic carcinoma cell lines stably expressing vector alone (FG-V), the b3 integrin subunit (FG-b3), or a b3 truncation mutant (FG-759x) probed with phospho-Src antibody. Ab was diluted 1:500.

Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the phospho-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-Src antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY215 of human Src.
Limitation:
This phospho-Src antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Chicken, Mouse, Rat, Xenopus, Zebrafish
Uniprot #:
P12931