FGFR2 Antibody

Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Titration of the FGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Fibroblast growth factor receptor 2 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed promotes activation of STAT1. [UniProt]

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 7-37 from the human protein was used as the immunogen for this FGFR2 antibody.

This FGFR2 antibody is available for research use only.

Purified

Human

Mouse

P21802