NSJ Bioreagents

MITF Antibody

Product Code:
 
NSJ-F41536
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the MITF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of MITF antibody at 1:2000 dilution + A375 lysate; Predicted molecular weight: 55-60 kDa.
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MITF antibody western blot analysis in K562 lysate. Predicted molecular weight: 55-60 kDa.
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Confocal immunofluorescent analysis of MITF antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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MITF antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram)
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Western blot testing of MITF antibody at 1:2000 dilution + A375 lysate; Predicted molecular weight: 55-60 kDa.
MITF antibody western blot analysis in K562 lysate. Predicted molecular weight: 55-60 kDa.
Confocal immunofluorescent analysis of MITF antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
MITF antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram)

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CodeSizePrice
NSJ-F41536-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41536-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000-2000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the MITF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-28 from the human protein was used as the immunogen for this MITF antibody.
Limitation:
This MITF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
O75030