NSJ Bioreagents

PARP Antibody

Product Code:
 
NSJ-F49614
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the PARP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of PARP antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (2).
2 / 3
PARP antibody western blot analysis in HeLa lysate
3 / 3
PARP antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of PARP antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (2).
PARP antibody western blot analysis in HeLa lysate
PARP antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F49614-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49614-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PARP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PARP is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 183-214 from the human protein was used as the immunogen for this PARP antibody.
Limitation:
This PARP antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
P09874