NSJ Bioreagents

FAS Antibody

Product Code:
 
NSJ-F51184
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the FAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of FAS antibody and T47D lysate. Soluble form ~26 kDa, membrane form ~40 kDa
2 / 3
Western blot analysis of FAS antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. Expected size ~40 kDa
3 / 3
Confocal immunofluorescent analysis of FAS antibody with T47D cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of FAS antibody and T47D lysate. Soluble form ~26 kDa, membrane form ~40 kDa
Western blot analysis of FAS antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. Expected size ~40 kDa
Confocal immunofluorescent analysis of FAS antibody with T47D cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

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CodeSizePrice
NSJ-F51184-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51184-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the FAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 185-211 from the human protein was used as the immunogen for this FAS antibody.
Limitation:
This FAS antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P25445