NSJ Bioreagents

KLF4 Antibody

Product Code:
 
NSJ-F48148
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:100. Note the highly specific localization of immunoreactivity to the nuclei.
2 / 4
Immunofluorescent analysis of U251 cells using KLF4 antibody; Ab was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
3 / 4
Fluorescent confocal image of HeLa cells stained with KLF4 antibody at 1:100. Note the highly specific localization of the immunoreactivity to the nuclei but not the cytoplasm.
4 / 4
Western blot analysis of KLF4 antibody and MDA-MB231, NCI-H460 lysate.Predicted molecular weight: 50-60 kDa + ~75 kDa
❮❯

Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:100. Note the highly specific localization of immunoreactivity to the nuclei.
Immunofluorescent analysis of U251 cells using KLF4 antibody; Ab was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Fluorescent confocal image of HeLa cells stained with KLF4 antibody at 1:100. Note the highly specific localization of the immunoreactivity to the nuclei but not the cytoplasm.
Western blot analysis of KLF4 antibody and MDA-MB231, NCI-H460 lysate.Predicted molecular weight: 50-60 kDa + ~75 kDa

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CodeSizePrice
NSJ-F48148-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48148-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Kruppel-like factor 4 (KLF4) is a transcription factor involved in both proliferation and differentiation in the colon.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 481-504 from the human protein was used as the immunogen for this KLF4 antibody.
Limitation:
This KLF4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
O43474