NSJ Bioreagents

LEF1 Antibody

Product Code:
 
NSJ-F41886
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Fluorescent confocal image of A431 cell stained with LEF1 antibody at 1:25. LEF1 immunoreactivity is localized to the nucleus strongly and cytoplasm weakly.~
2 / 2
LEF1 antibody western blot analysis in MDA-MB435 lysate.
❮❯

Fluorescent confocal image of A431 cell stained with LEF1 antibody at 1:25. LEF1 immunoreactivity is localized to the nucleus strongly and cytoplasm weakly.~
LEF1 antibody western blot analysis in MDA-MB435 lysate.

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CodeSizePrice
NSJ-F41886-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41886-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the LEF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Lymphoid enhancer-binding factor 1 participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 10-37 from the human protein was used as the immunogen for this LEF1 antibody.
Limitation:
This LEF1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
Q9UJU2