NANOG Antibody

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NSJ Bioreagents
Product Code: NSJ-F44249
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F44249-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44249-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Fluorescent confocal image of SY5Y cells stained with NANOG antibody at 1:200. NANOG immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
2 / 3
Western blot analysis of NANOG antibody and HepG2 lysate. Predicted molecular weight: 35-45 kDa.
3 / 3
Western blot analysis of NANOG antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2). Predicted molecular weight: 35-45 kDa.
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Fluorescent confocal image of SY5Y cells stained with NANOG antibody at 1:200. NANOG immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
Western blot analysis of NANOG antibody and HepG2 lysate. Predicted molecular weight: 35-45 kDa.
Western blot analysis of NANOG antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2). Predicted molecular weight: 35-45 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 267-292 from the human protein was used as the immunogen for this NANOG antibody.
Limitation:
This NANOG antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9H9S0